ABSTRACT
Abstract Fluorescent nanodiamond (FND) has been used for long-term cell labeling and in vivo cell tracking because they have good at photostability and biocompatibility. In this study, we evaluate the effect of fluorescent nanodiamond labeling on in vitro culture and differentiation of human umbilical cord mesenchymal stem cells (hUCMSCs) into hepatocyte-like cells (HLCs). For hepatic differentiation of hUCMSCs, cells were induced with human hepatocyte growth factor, nicotinamide and Dexamethasone. FND was supplied in two experimental groups with 20 μg/mL and 100 μg/mL in 2 hours. The cell was assessed for FND uptake by laser scan microscopy and flow cytometry methods. The effect of FND on hUCMSCs was evaluated by the cell viability and growth assays as well as the differentiation throughout of morphology alterations or gene expression of anfa-fetoprotein, albumin, and hepatocyte nuclear factor 4α. The results showed that the labeling of hUCMSCs is efficient and easy and there was significant cellular uptake of FND. We did not observe any negative impacts of FND to the cell viability and growth. FND can be utilized for the long-term labeling and tracking of hUCSCs and HLCs in vivo studies.
Subject(s)
Humans , Umbilical Cord/cytology , Cell Differentiation , Hepatocytes/cytology , Mesenchymal Stem Cells/cytology , Cell Survival , Reverse Transcriptase Polymerase Chain ReactionABSTRACT
Objective To study the protective effects of umbilical cord stem cells on cardiac injury in severely burned rats. Methods 30 SD rats were randomly divided into sham group ,burn group and stem cells treatment group. The severely burned head ,neck and back of rat model of 35%TBSAⅢdegree was eatabished in burn and stem cells treatment groups. The blood and cardiac tissues were collected at 24 hours after stem cells transplantation. The distribution patterns of umbilical cord stem cells in cardiac tissues ,and infiltrations of neutro-phils and macrophages in cardiac tissues were detected by immunohistochemistry staining method. The cardiac structures of rats were evaluated by HE staining. The levels of Troponin I ,Creatine Kinase and Creatine Kinase-MB in serum were detected by the blood biochemical automatic analyzer. Results The umbilical cord stem cells migrated into the injured cardiac tissues and mainly concentrated in capillaries of heart. The umbilical cord stem cells significantly decreased neutrophils and macrophages infiltration of the cardiac tissues ,and markedly alleviated structure damages of the cardiac tissues in severely burned rats. Furthermore ,the levels of Troponin I ,Creatine Kinase,Creatine Kinase-MB of burn group were significantly higher than that of sham group,but those of stem cells treatment group were markedly lower than that of burn group (P < 0.05). Conclution The umbilical cord stem cells transplantation could effectively reduce inflammatory cells infiltration of the cardiac tissues,then protected the structure and function of the heart in severely burned rats.
ABSTRACT
Objective To study the protective effects of umbilical cord stem cells on cardiac injury in severely burned rats. Methods 30 SD rats were randomly divided into sham group ,burn group and stem cells treatment group. The severely burned head ,neck and back of rat model of 35%TBSAⅢdegree was eatabished in burn and stem cells treatment groups. The blood and cardiac tissues were collected at 24 hours after stem cells transplantation. The distribution patterns of umbilical cord stem cells in cardiac tissues ,and infiltrations of neutro-phils and macrophages in cardiac tissues were detected by immunohistochemistry staining method. The cardiac structures of rats were evaluated by HE staining. The levels of Troponin I ,Creatine Kinase and Creatine Kinase-MB in serum were detected by the blood biochemical automatic analyzer. Results The umbilical cord stem cells migrated into the injured cardiac tissues and mainly concentrated in capillaries of heart. The umbilical cord stem cells significantly decreased neutrophils and macrophages infiltration of the cardiac tissues ,and markedly alleviated structure damages of the cardiac tissues in severely burned rats. Furthermore ,the levels of Troponin I ,Creatine Kinase,Creatine Kinase-MB of burn group were significantly higher than that of sham group,but those of stem cells treatment group were markedly lower than that of burn group (P < 0.05). Conclution The umbilical cord stem cells transplantation could effectively reduce inflammatory cells infiltration of the cardiac tissues,then protected the structure and function of the heart in severely burned rats.